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Hank's Balanced Salt Solution (HBSS) Preparation and Recipe

To prepare L  Hank's Balanced Salt Solution (HBSS)

Table 1. Recipe

NameAmountConcentrationFormula/M.W.CAS #
Sodium chloride7.999 g0.136 MNaCl/58.447647-14-5
Potassium Chloride0.4 g5.365 mMKCl/74.557447-40-7
0.14 g1.261 mMCaCl2/110.9810043-52-4
0.048 g0.405 mMMgSO4/120.377487-88-9
0.046 g0.491 mMMgCl2/95.217786-30-3
0.047 g0.337 mMNa2HPO4/141.967558-79-4
Potassium dihydrogen phosphate0.06 g0.44 mMKH2PO4/136.097778-77-0
Glucose1 g5.55 mMC6H12O6/180.1650-99-7
Sodium bicarbonate0.35 g4.166 mMNaHCO3/84.01144-55-8
  1. Prepare 0.8 L of distilled water in a suitable container.
  2. Add 7.999 g of Sodium chloride to the solution.
  3. Add 0.4 g of Potassium Chloride to the solution.
  4. Add 0.14 g of Calcium Chloride to the solution.
  5. Add 0.048 g of Magnesium sulfate anhydrous to the solution.
  6. Add 0.046 g of Magnesium chloride to the solution.
  7. Add 0.047 g of Sodium phosphate dibasic to the solution.
  8. Add 0.06 g of Potassium dihydrogen phosphate to the solution.
  9. Add 1 g of Glucose to the solution.
  10. Add 0.35 g of Sodium bicarbonate to the solution.
  11. Add distilled water until the volume is 1 L.
  12. Sterilize and refrigerate