To prepare L Gentamicin Stock Solution
Table 1. Recipe
Name | Amount | Concentration | Formula/M.W. | CAS # |
---|---|---|---|---|
Gentamicin | 20 g | 20 mg/mL | C21H43N5O7/477.6 | 1403-66-3 |
- Prepare 0.8 L of distilled water in a suitable container.
- Add 20 g of Gentamicin to the solution.
- Add distilled water until the volume is 1 L.
- Prewet a 0.22 µm syringe filter by drawing through 5-10 ml of sterile H2O and discard water. Sterilize Gentamicin Stock through the prepared 0.22 µm syringe filter. Stock may be kept at -20°C for 1 year. Working Conc. = 20 µg/mL