To prepare L Ethidium Bromide Solution
Table 1. Recipe
Name | Amount | Concentration | Formula/M.W. | CAS # |
---|---|---|---|---|
Ethidium bromide | 10 g | 0.025 M | C21H20BrN3/394.32 | 1239-45-8 |
- Prepare 0.8 L of distilled water in a suitable container.
- Add 10 g of Ethidium bromide to the solution.
- Add distilled water until the volume is 1 L.
- Stir on a magnetic stirrer for several hours to ensure that the dye has dissolved. Wrap the container in aluminum foil or transfer the 10 mg/mL solution to a dark bottle and store at room temperature.