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Embryonic Medium Preparation and Recipe

To prepare L  Embryonic Medium

Table 1. Recipe

NameAmountConcentrationFormula/M.W.CAS #
Sodium chloride0.876 g0.015 MNaCl/58.447647-14-5
Potassium Chloride0.037 g0.5 mMKCl/74.557447-40-7
0.11 g1 mMCaCl2/110.9810043-52-4
0.246 g1 mMMgSO4·7H2O/246.4710034-99-8
Potassium dihydrogen phosphate0.02 g0.15 mMKH2PO4/136.097778-77-0
0.017 g0.05 mMNa2HPO4·12H2O/358.1410039-32-4
Sodium bicarbonate0.058 g0.7 mMNaHCO3/84.01144-55-8
  1. Prepare 0.8 L of distilled water in a suitable container.
  2. Add 0.876 g of Sodium chloride to the solution.
  3. Add 0.037 g of Potassium Chloride to the solution.
  4. Add 0.11 g of Calcium Chloride to the solution.
  5. Add 0.246 g of Magnesium sulfate heptahydrate to the solution.
  6. Add 0.02 g of Potassium dihydrogen phosphate to the solution.
  7. Add 0.017 g of Sodium phosphate dibasic dodecahydrate to the solution.
  8. Add 0.058 g of Sodium bicarbonate to the solution.
  9. Adjust the pH to 7.0-7.5.
  10. Add distilled water until the volume is 1 L.