Digestion Medium Preparation and Recipe

To prepare L  Digestion Medium

Table 1. Recipe

1. Prepare 0.8 L of distilled water in a suitable container.
2. Add 23.376 g of Sodium chloride to the solution.
3. Add 26.429 g of Magnesium chloride hexahydrate to the solution.
4. Add 5.422 g of Magnesium sulfate heptahydrate to the solution.
5. Add 11.927 g of Potassium Chloride to the solution.
6. Add 0.221 g of Calcium Chloride to the solution.
7. Add 1.952 g of 2-(N-morpholino)ethanesulfonic acid to the solution.
8. Adjust the pH to 6.5.
9. Add distilled water until the volume is 1 L.
10. Filter through a 0.2-μm filter and store at 4°C. Just before use, add 1% (w/v) cellulose and 3 U/mL alginate lyase (Boyen et al. 1990).