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Chelation Medium Preparation and Recipe

To prepare L  Chelation Medium

Table 1. Recipe

NameAmountConcentrationFormula/M.W.CAS #
Sodium chloride40.907 g0.7 MNaCl/58.447647-14-5
6.099 g0.03 MMgCl2·6H2O/203.37791-18-6
7.394 g0.03 MMgSO4·7H2O/246.4710034-99-8
Potassium Chloride1.49 g0.02 MKCl/74.557447-40-7
Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid7.607 g0.02 MC14H24N2O10/380.3567-42-5
  1. Prepare 0.8 L of distilled water in a suitable container.
  2. Add 40.907 g of Sodium chloride to the solution.
  3. Add 6.099 g of Magnesium chloride hexahydrate to the solution.
  4. Add 7.394 g of Magnesium sulfate heptahydrate to the solution.
  5. Add 1.49 g of Potassium Chloride to the solution.
  6. Add 7.607 g of Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid to the solution.
  7. Adjust the pH to 5.5.
  8. Add distilled water until the volume is 1 L.
  9. Autoclave or filter through a 0.2-μm membrane. Store at 4°C.