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Ca and Mg-free Dulbeccos phosphate-buffered saline (CMF-DPBS) Preparation and Recipe

To prepare L  Ca and Mg-free Dulbeccos phosphate-buffered saline (CMF-DPBS)

Table 1. Recipe

NameAmountConcentrationFormula/M.W.CAS #
Sodium chloride8.006 g0.137 MNaCl/58.447647-14-5
Potassium Chloride0.201 g2.7 mMKCl/74.557447-40-7
1.149 g8.1 mMNa2HPO4/141.967558-79-4
Potassium dihydrogen phosphate0.149 g1.1 mMKH2PO4/136.097778-77-0
0.047 g0.5 mMMgCl2/95.217786-30-3
0.099 g0.9 mMCaCl2/110.9810043-52-4
  1. Prepare 0.8 L of distilled water in a suitable container.
  2. Add 8.006 g of Sodium chloride to the solution.
  3. Add 0.201 g of Potassium Chloride to the solution.
  4. Add 1.149 g of Sodium phosphate dibasic to the solution.
  5. Add 0.149 g of Potassium dihydrogen phosphate to the solution.
  6. Add 0.047 g of Magnesium chloride to the solution.
  7. Add 0.099 g of Calcium Chloride to the solution.
  8. Add distilled water until the volume is 1 L.