To prepare L Bradford Stock Solution
Table 1. Recipe
Name | Amount | Concentration | Formula/M.W. | CAS # |
---|---|---|---|---|
Ethanol | 0.316 L | 31.666% v/v | CH3CH2OH/46.07 | 64-17-5 |
Phosphoric acid | 0.586 L | 58.666% v/v | H3PO4/98 | 7664-38-2 |
Coomassie brilliant blue G-250 | 1.209 g | 1.41 mM | C47H48N3NaO7S2/858.05 | 6104-58-1 |
- Prepare 0.8 L of distilled water in a suitable container.
- Add 0.316 L of Ethanol to the solution.
- Add 0.586 L of Phosphoric acid to the solution.
- Add 1.209 g of Coomassie brilliant blue G-250 to the solution.
- Add distilled water until the volume is 1 L.
- The stock solution is stable indefinitely when stored in a brown glass bottle at room temperature.