To prepare L Bath Solution (Ca2+/Mg2+ Free)
Table 1. Recipe
| Name | Amount | Concentration | Formula/M.W. | CAS # |
|---|---|---|---|---|
| Potassium hydroxide | 7.855 g | 0.14 M | KOH/56.11 | 1310-58-3 |
| Potassium Chloride | 0.149 g | 2 mM | KCl/74.55 | 7447-40-7 |
| Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid | 1.901 g | 5 mM | C14H24N2O10/380.35 | 67-42-5 |
| HEPES(N-2-Hydroxyethylpiperazine-N'-2-ethanesulfonic acid) | 4.766 g | 0.02 M | C8H18N2O4S/238.3 | 7365-45-9 |
| Methanesulfonic Acid | 13.455 g | 0.14 M | CH4O3S/96.11 | 75-75-2 |
- Prepare 0.8 L of distilled water in a suitable container.
- Add 7.855 g of Potassium hydroxide to the solution.
- Add 0.149 g of Potassium Chloride to the solution.
- Add 1.901 g of Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid to the solution.
- Add 4.766 g of HEPES(N-2-Hydroxyethylpiperazine-N'-2-ethanesulfonic acid) to the solution.
- Add 13.455 g of Methanesulfonic Acid to the solution.
- Adjust the pH to 7.1–7.2.
- Add distilled water until the volume is 1 L.
- Use methanesulfonic acid to adjust the pH to 7.1–7.2. Store for up to 3 years at room temperature (22°C–24°C).