Anesthetic Solution (Buffered MS222) is commonly used to temporarily immobilize fish and amphibians.
To prepare L Anesthetic (MS222) Solution
Table 1. Recipe
Name | Amount | Concentration | Formula/M.W. | CAS # |
---|---|---|---|---|
MS222(3-aminobenzoic acid ethyl ester) | 1 g | 3.827 mM | C9H11NO2·CH4O3S/261.29 | 886-86-2 |
Sodium bicarbonate | 0.7 g | 8.332 mM | NaHCO3/84.01 | 144-55-8 |
- Prepare 0.8 L of distilled water in a suitable container.
- Add 1 g of MS222(3-aminobenzoic acid ethyl ester) to the solution.
- Add 0.7 g of Sodium bicarbonate to the solution.
- Add distilled water until the volume is 1 L.
- The resulting pH should be ∼7.0. Store in a foil-covered container at 4°C for up to 1 month. Warm to room temperature before use.