To prepare L 10x TB (Tris-Borate) electrophoresis buffer
Table 1. Recipe
Name | Amount | Concentration | Formula/M.W. | CAS # |
---|---|---|---|---|
Tris base | 107.814 g | 0.89 M | C4H11NO3/121.14 | 77-86-1 |
Boric acid | 55.037 g | 0.89 M | H3BO3/61.84 | 10043-35-3 |
- Prepare 0.8 L of distilled water in a suitable container.
- Add 107.814 g of Tris base to the solution.
- Add 55.037 g of Boric acid to the solution.
- Add distilled water until the volume is 1 L.