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Product Name
ZIPK-Phospho 311Ser antibody
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Description
ZIPK-Phospho 311Ser Rabbit Polyclonal antibody. Positive IHC detected in human breast cancer tissue. Positive WB detected in HEK-293 cells, Transfected HEK-293 cells. Positive IP detected in HEK-293 cells. Observed molecular weight by Western-blot: 52.5kd
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
DAP kinase 3 antibody; DAP like kinase antibody; DAPK3 antibody; Dlk antibody; FLJ36473 antibody; ZIP antibody; ZIP kinase antibody; ZIPK antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_001348). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:117063(ZIPK-Phospho 311Ser antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:117063(ZIPK-Phospho 311Ser antibody) at dilution of 1:50 (under 10x lens)
IP Result of anti-ZIPK-Phospho 311Ser (IP:Catalog No:117063, 4ug; Detection:Catalog No:117063 1:300) with HEK-293 cells lysate 3200ug.
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Background
DAPK3(Death-associated protein kinase 3) is also named as ZIPK(Zipper-interacting protein kinase),Dlk(DAP-like kinase). It is a member of the death-associated protein kinase family associated with apoptosis in nonmuscle cells where it phosphorylates myosin regulatory light chain (RLC) to promote membrane blebbing. DAPK3 mRNA and protein are abundant in heart tissue and isolated ventricular neonatal rat cardiac myocytes(PMID:20038585). It has 2 isoforms produced by alternative splicing,named as ZIPK-L and ZIPK-S. This antibody is specific to ZIPK-Phospho 311Ser.
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References
- Weitzel DH, Chambers J, Haystead TA. Phosphorylation-dependent control of ZIPK nuclear import is species specific. Cellular signalling. 23(1):297-303. 2011.
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