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Product Name
ZIP8 antibody
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Description
ZIP8 Rabbit Polyclonal antibody. Positive IHC detected in human placenta tissue, human kidney tissue. Positive IF detected in HepG2 cells. Positive IP detected in mouse liver tissue. Positive WB detected in mouse lung tissue, mouse heart tissue, mouse liver tissue, mouse pancreas tissue, mouse thymus tissue, U-937 cells. Observed molecular weight by Western-blot: 42-46; 75-90 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
BIGM103 antibody; PP3105 antibody; SLC39A8 antibody; ZIP 8 antibody; ZIP8 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ZIP8 recombinant protein (Accession Number: NM_022154). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse lung tissue were subjected to SDS PAGE followed by western blot with Catalog No:117062(SLC39A8 antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human placenta using Catalog No:117062(SLC39A8 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human placenta using Catalog No:117062(SLC39A8 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using SLC39A8 antibody Catalog No:117062 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-SLC39A8 (IP:Catalog No:117062, 4ug; Detection:Catalog No:117062 1:500) with mouse liver tissue lysate 6000ug.
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Background
SLC39A8 also known as ZIP8 belongs to the ZIP family of metal ion transporters which function to transport zinc and/or other metal ion substrates from the extracellular space or organellar lumen into the cytoplasm. Recently it was found that Zip8 expression is upregulated in human monocytes in response to LPS, TNF-α, and live bacteria, facilitating cytoprotection during the early inflammation. Besides zinc ZIP8 can also transport cadmium and manganese efficiently. It is predicted that ZIP8 contains 3 potential N-linked glycosylation sites and is subject to glycosylation, which may account for the presences of multiple molecular weights, such as 43 kDa, 49 kDa, 60 kDa, 75-90 kDa, 150 kDa, and 200 kDa.
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References
- Fujishiro H, Yoshida M, Nakano Y, Himeno S. Interleukin-6 enhances manganese accumulation in SH-SY5Y cells: implications of the up-regulation of ZIP14 and the down-regulation of ZnT10. Metallomics : integrated biometal science. 6(4):944-9. 2014.
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- Grubman A, Pollari E, Duncan C. Deregulation of biometal homeostasis: the missing link for neuronal ceroid lipofuscinoses? Metallomics : integrated biometal science. 6(4):932-43. 2014.
- Yin XF, Jiang LB, Ma YQ. Decreased Zn(2+) Influx Underlies the Protective Role of Hypoxia in Rat Nucleus Pulposus Cells. Biological trace element research. 168(1):196-205. 2015.
- Sun Q, Zhong W, Zhang W. Zinc deficiency mediates alcohol-induced apoptotic cell death in the liver of rats through activating ER and mitochondrial cell death pathways. American journal of physiology. Gastrointestinal and liver physiology. 308(9):G757-66. 2015.
- Amos-Kroohs RM, Bloor CP, Qureshi MA, Vorhees CV, Williams MT. Effects of developmental exposure to manganese and/or low iron diet: Changes to metal transporters, sucrose preference, elevated zero-maze, open-field, and locomotion in response to fenfluramine, amphetamine, and MK-801. Toxicology reports. 2:1046-1056. 2015.
- Kanninen KM, Grubman A, Caragounis A. Altered biometal homeostasis is associated with CLN6 mRNA loss in mouse neuronal ceroid lipofuscinosis. Biology open. 2(6):635-46. 2013.
- Kim JH, Jeon J, Shin M. Regulation of the catabolic cascade in osteoarthritis by the zinc-ZIP8-MTF1 axis. Cell. 156(4):730-43. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"