ZIP peptide

ZIP peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

Myrisitic Acid-SIYRRGARRWRKL-OH

Myrisitic Acid-Ser-Ile-Tyr-Arg-Arg-Gly-Ala-Arg-Arg-Trp-Arg-Lys-Leu-OH

13

95.22%

C₉₀H₁₅₄N₃₀O₁₇

1928.37

Synthetic

Powder

ZIP, or Zeta inhibitory peptide, is a cell permeable pseudo-substrate inhibitor of protein kinase Mζ (PKMζ), a constitutively active, atypical PKC isozyme involved in LTP maintenance. ZIP selectively blocks PKMζ induced synaptic potentiation in hippocampal slices in vitro and is widely used to investigate memory in a variety of learning tasks, including spatial sensitization, pain sensitization, fear-associated memories, conditioned taste aversion and development of locomotor sensitization in rodents. However, LTP and LTM are unaffected in both constitutive and conditional PKMζ knock-out mice, yet both are still impaired by ZIP application, suggesting another mechanism of action which may involve ionotropic glutamate AMPA receptors (AMPARs).

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Serrano et al (2005) Persistent phosphorylation by protein kinase Mz maintains late-phase long-term potentiation. J.Neurosci. 25 1979 PMID: 15728837
• LeBlancq et al (2016) ZIP It: Neural Silencing Is an Additional Effect of the PKM-Zeta Inhibitor Zeta-Inhibitory Peptide. J. Neuroscience 36 (23) 6193 DOI: 10.1523/JNEUROSCI.4563-14.2016
• Bingor et al (2020) Zeta Inhibitory Peptide attenuates learning and memory by inducing NO-mediated downregulation of AMPA receptors. Nat Commun 11 3688 https://doi.org/10.1038/s41467-020-17484-y

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.