Z-Tyr-Lys-Arg-pNA peptide

Z-Tyr-Lys-Arg-pNA peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

Cbz-YKR-pNA

Cbz-Tyr-Lys-Arg-pNA

3

95.68%

C₃₅H₄₅N₉O₈

719.78

108318-36-1

194771

Synthetic

Powder

Z-Tyr-Lys-Arg-pNA (benzyloxycarbonyl-Tyr-Lys-Arg-p-nitroanilide) is a chromogenic peptide substrate used to assess the protease activity in the spent culture medium (SCM) of Kluyveromyces lactis mutant strains. Yapsin family proteases have a preference for cleavage of proteins at basic amino acids, and the Lys-Arg motif within this substrate is a well-characterized cleavage site for yapsin proteases. By measuring the hydrolysis of this substrate in the SCM, the researchers could determine the protease activity attributable to yapsin family proteases in each mutant strain.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Ganatra MB, Vainauskas S, Hong JM, Taylor TE, Denson JP, Esposito D, Read JD, Schmeisser H, Zoon KC, Hartley JL, Taron CH. A set of aspartyl protease-deficient strains for improved expression of heterologous proteins in Kluyveromyces lactis. FEMS Yeast Res. 2011 Mar;11(2):168-78. doi: 10.1111/j.1567-1364.2010.00703.x. Epub 2010 Dec 17. PMID: 21166768; PMCID: PMC3041862.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.