Z-DEVD-AFC peptide
Not For Human Use, Lab Use Only.
Cat.#: 319646
Special Price 229.0 USD
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Product Name
Z-DEVD-AFC peptide
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Documents
Batch to batch variation of the purity
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Sequence Shortening
Cbz-DEVD-AFC
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Sequence
Cbz-Asp-Glu-Val-Asp-AFC
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Length (aa)
4
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Peptide Purity (HPLC)
95.92%
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Molecular Formula
C36H38F3N5O14
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Molecular Weight
821.7
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CAS No.
1135416-11-3
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PubChem CID
25108569
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Source
Synthetic
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Form
Powder
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Description
Z-DEVD-AFC is a cell-permeant substrate for caspase-3, which causes a shift in fluorescence uponcleavage of the AFC fluorophore. Z-DEVD-AFC can be used to detect caspase-3-like enzymes activity.
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Storage Guidelines
Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides
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References
- Santiago AR, Cristóvão AJ, Santos PF, Carvalho CM, Ambrósio AF. High glucose induces caspase-independent cell death in retinal neural cells. Neurobiol Dis. 2007 Mar;25(3):464-72. doi: 10.1016/j.nbd.2006.10.023. Epub 2006 Dec 16. PMID: 17239603.
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About TFA salt
Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.
TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.
TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.
In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.
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Molar Concentration Calculator
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Dilution Calculator
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Percent Concentration Calculator
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)
Related Products / Services
• Peptide Services: NovoPro's peptide synthesis services include standard chemical peptide synthesis, peptide modification, peptide libraries, and recombinant peptide expression.
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• Peptide Modifications: NovoPro offers a wide range of peptide modification services including isotope labeling (2H, 15N, and 13C), multiple disulfide bonds, multiple phosphorylations, KLH, BSA, ovalbumin, amidation, acetylation, biotin, FITC, etc.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"