Z-Ala-Ala-Asn-AMC peptide
Not For Human Use, Lab Use Only.
Cat.#: 319624
Special Price 259.0 USD
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Product Name
Z-Ala-Ala-Asn-AMC peptide
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Documents
Batch to batch variation of the purity
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Sequence Shortening
Cbz-AAN-AMC
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Sequence
Cbz-Ala-Ala-Asn-AMC
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Length (aa)
3
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Peptide Purity (HPLC)
95.51%
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Molecular Formula
C28H31N5O8
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Molecular Weight
565.57
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CAS No.
149697-16-5
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PubChem CID
90470169
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Source
Synthetic
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Form
Powder
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Description
Z-Ala-Ala-Asn-AMC (Cbz-Ala-Ala-Asn-AMC) is the legumain substrate. Legumain is a lysosomal protease that specifically cleaves asparaginyl bonds. It has been reported to be overexpressed in the majority of human solid tumors and is associated with enhanced tissue invasion and metastases, making it a promising candidate for prodrug therapy. CBZ-Ala-Ala-Asn-AMC is an important tool for studying the activity of legumain and its potential as a target for prodrug therapy.
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Storage Guidelines
Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides
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References
- Stern L, Perry R, Ofek P, Many A, Shabat D, Satchi-Fainaro R. A novel antitumor prodrug platform designed to be cleaved by the endoprotease legumain. Bioconjug Chem. 2009 Mar 18;20(3):500-10. doi: 10.1021/bc800448u. PMID: 19196156.
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About TFA salt
Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.
TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.
TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.
In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.
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Molar Concentration Calculator
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Dilution Calculator
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Percent Concentration Calculator
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)
Related Products / Services
• Peptide Services: NovoPro's peptide synthesis services include standard chemical peptide synthesis, peptide modification, peptide libraries, and recombinant peptide expression.
• Standard Peptide Synthesis: NovoPro offers quality peptides at the most competitive prices in the industry, starting at $3.20 per amino acid. NovoPro provides PepBox – Automatic Quote Tool for online price calculation.
• Peptide Modifications: NovoPro offers a wide range of peptide modification services including isotope labeling (2H, 15N, and 13C), multiple disulfide bonds, multiple phosphorylations, KLH, BSA, ovalbumin, amidation, acetylation, biotin, FITC, etc.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"