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Product Name
YBX1 antibody
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Description
YBX1 Rabbit Polyclonal antibody. Positive IP detected in Jurkat cells. Positive WB detected in MCF7 cells, HEK-293 cells, HeLa cells, Jurkat cells, mouse spleen tissue. Positive IHC detected in human placenta tissue, human cervical cancer tissue. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 50 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
BP 8 antibody; CBF A antibody; CSDA2 antibody; CSDB antibody; DBPB antibody; DNA binding protein B antibody; EFI A antibody; Enhancer factor I subunit A antibody; MDR NF1 antibody; NSEP 1 antibody; NSEP1 antibody; Y box binding protein 1 antibody; Y box transcription factor antibody; YB 1 antibody; YB1 antibody; YBX1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of YBX1 recombinant protein (Accession Number: NM_004559). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:116882(YBX1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human placenta using Catalog No:116882(YBX1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human placenta using Catalog No:116882(YBX1 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-YBX1 (IP:Catalog No:116882, 4ug; Detection:Catalog No:116882 1:500) with Jurkat cells lysate 3080ug.
Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:116882(YBX1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Y box binding protein 1(YBX1) is one of cis-acting elements which has a role in regulation of the expression of HLA class II genes. YBX1 is great of importance in regulation of PTP1B expression. It can bind to splice sites mediate pre-mRNA alternative splicing regulation. Also it involves in the regulation of translation by modulation of the interaction between the mRNA and eukaryotic initiation factors. Its transcriptional activity on the multidrug resistance gene MDR1 is enhanced in presence of the APEX1 acetylated form at 'Lys-6' and 'Lys-7'. The secreted form acts as an extracellular mitogen and stimulates cell migration and proliferation
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"