WT1C peptide

WT1C peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-ALLPAVPSL-OH

H-Ala-Leu-Leu-Pro-Ala-Val-Pro-Ser-Leu-OH

9

95.02%

C₄₂H₇₃N₉O₁₁

880.08

Synthetic

Powder

WT1C is a HLA-A*02:01-restricted peptide. WT1C (ALLPAVPSL) showed a high binding score for HLA-A*02:01 but elicited low frequencies of T-cells in individuals. WT1C can be processed from WT1 and presented on HLA-A*02, but its cognate TCR are eliminated during the course of negative selection of thymocytes in nonresponding individuals. WT1C may represent a promising target for TCRm-Ab development.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Kurosawa, N, Midorikawa, A, Ida, K, Fudaba, YW, Isobe, M. Development of a T-cell receptor mimic antibody targeting a novel Wilms tumor 1-derived peptide and analysis of its specificity. Cancer Sci. 2020; 111: 3516- 3526. https://doi.org/10.1111/cas.14602

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.