WT1C peptide
Not For Human Use, Lab Use Only.
Cat.#: 319242
Special Price 94.2 USD
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Product Name
WT1C peptide
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Documents
Batch to batch variation of the purity
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Sequence Shortening
H-ALLPAVPSL-OH
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Sequence
H-Ala-Leu-Leu-Pro-Ala-Val-Pro-Ser-Leu-OH
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Length (aa)
9
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Peptide Purity (HPLC)
95.02%
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Molecular Formula
C42H73N9O11
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Molecular Weight
880.08
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Source
Synthetic
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Form
Powder
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Description
WT1C is a HLA-A*02:01-restricted peptide. WT1C (ALLPAVPSL) showed a high binding score for HLA-A*02:01 but elicited low frequencies of T-cells in individuals. WT1C can be processed from WT1 and presented on HLA-A*02, but its cognate TCR are eliminated during the course of negative selection of thymocytes in nonresponding individuals. WT1C may represent a promising target for TCRm-Ab development.
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Storage Guidelines
Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides
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References
- Kurosawa, N, Midorikawa, A, Ida, K, Fudaba, YW, Isobe, M. Development of a T-cell receptor mimic antibody targeting a novel Wilms tumor 1-derived peptide and analysis of its specificity. Cancer Sci. 2020; 111: 3516- 3526. https://doi.org/10.1111/cas.14602
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About TFA salt
Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.
TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.
TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.
In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.
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Molar Concentration Calculator
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Dilution Calculator
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Percent Concentration Calculator
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)
Related Products / Services
• Peptide Services: NovoPro's peptide synthesis services include standard chemical peptide synthesis, peptide modification, peptide libraries, and recombinant peptide expression.
• Standard Peptide Synthesis: NovoPro offers quality peptides at the most competitive prices in the industry, starting at $3.20 per amino acid. NovoPro provides PepBox – Automatic Quote Tool for online price calculation.
• Peptide Modifications: NovoPro offers a wide range of peptide modification services including isotope labeling (2H, 15N, and 13C), multiple disulfide bonds, multiple phosphorylations, KLH, BSA, ovalbumin, amidation, acetylation, biotin, FITC, etc.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"