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Product Name
WDR24 antibody
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Description
WDR24 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, mouse brain tissue. Positive IF detected in Hela cells, HepG2 cells. Positive IHC detected in human ovary tissue, human colon tissue. Observed molecular weight by Western-blot: 102 kDa,88 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
C16orf21 antibody; DKFZp434F054 antibody; JFP7 antibody; WD repeat domain 24 antibody; WDR24 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of WDR24 recombinant protein (Accession Number: NM_032259). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:116861(WDR24 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human ovary using Catalog No:116861(WDR24 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human ovary using Catalog No:116861(WDR24 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of Hela cells, using WDR24 antibody Catalog No:116861 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-WDR24 (IP:Catalog No:116861, 4ug; Detection:Catalog No:116861 1:500) with HeLa cells lysate 3200ug.
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Background
WDR24 is a component of the GATOR2 complex which has five known subunits (WDR24, WDR59, Mios, Sec13, and Seh1L). GATOR2 complex positively regulates mTORC1 signaling by acting upstream of or in parallel to GATOR1, which is a GTPase-activating protein (GAP) for RagA or RagB and an inhibitor of the amino-acid-sensing pathway. (PMID: 23723238; 25263562)
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