WDR24 antibody

Cat.#: 116861

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Product Information

  • Product Name
    WDR24 antibody
  • Documents
  • Description
    WDR24 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, mouse brain tissue. Positive IF detected in Hela cells, HepG2 cells. Positive IHC detected in human ovary tissue, human colon tissue. Observed molecular weight by Western-blot: 102 kDa,88 kDa
  • Tested applications
    ELISA, WB, IHC, IF, IP
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    C16orf21 antibody; DKFZp434F054 antibody; JFP7 antibody; WD repeat domain 24 antibody; WDR24 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of WDR24 recombinant protein (Accession Number: NM_032259). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

    IF: 1:10-1:100

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:116861(WDR24 antibody) at dilution of 1:500

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:116861(WDR24 antibody) at dilution of 1:500

    Immunohistochemical of paraffin-embedded human ovary using Catalog No:116861(WDR24 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human ovary using Catalog No:116861(WDR24 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human ovary using Catalog No:116861(WDR24 antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human ovary using Catalog No:116861(WDR24 antibody) at dilution of 1:50 (under 40x lens)

    Immunofluorescent analysis of Hela cells, using WDR24 antibody Catalog No:116861 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    Immunofluorescent analysis of Hela cells, using WDR24 antibody Catalog No:116861 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    IP Result of anti-WDR24 (IP:Catalog No:116861, 4ug; Detection:Catalog No:116861 1:500) with HeLa cells lysate 3200ug.

    IP Result of anti-WDR24 (IP:Catalog No:116861, 4ug; Detection:Catalog No:116861 1:500) with HeLa cells lysate 3200ug.

  • Background
    WDR24 is a component of the GATOR2 complex which has five known subunits (WDR24, WDR59, Mios, Sec13, and Seh1L). GATOR2 complex positively regulates mTORC1 signaling by acting upstream of or in parallel to GATOR1, which is a GTPase-activating protein (GAP) for RagA or RagB and an inhibitor of the amino-acid-sensing pathway. (PMID: 23723238; 25263562)

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"