W379 peptide

W379 peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-RRRWWWWV-OH

H-Arg-Arg-Arg-Trp-Trp-Trp-Trp-Val-OH

8

99.14%

C₆₇H₈₇N₂₁O₉

1330.54

Synthetic

Powder

W379 is a LL-37 mimetic peptide. Compared to LL-37 (37 residues), W379 comprises only eight amino acids (sequence: RRRWWWWV). W379 maintains a broad-spectrum antimicrobial activity and simultaneously exhibits superiority in several aspects, including a unique amphipathic structure and a significant reduction in production cost. W379 peptide can enhance skin cell migration in an in vitro wound-healing assay. In vivo studies indicated patterned macrochannels in nanofiber aerogels can greatly enhance vascularized granulation tissue formation and the addition of W379 can promote not only vascularized granulation tissue formation but also re-epithelialization. The enhanced wound healing by W379 could be due to the activation of the p38 MAPK signaling pathway.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• John JV, Sharma NS, Tang G, Luo Z, Su Y, Weihs S, Shahriar SMS, Wang G, McCarthy A, Dyke J, Zhang YS, Khademhosseini A, Xie J. Nanofiber Aerogels with Precision Macrochannels and LL-37-Mimic Peptides Synergistically Promote Diabetic Wound Healing. Adv Funct Mater. 2023 Jan 3;33(1):2206936. doi: 10.1002/adfm.202206936. Epub 2022 Oct 31. PMID: 36714167; PMCID: PMC9881731.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.