VPV peptide

VPV peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-VPV-OH

H-Val-Pro-Val-OH

3

95.46%

C₁₅H₂₇N₃O₄

313.39

Synthetic

Powder

VPV is a DPP-IV(Dipeptidyl peptidase IV) inhibitory peptide. VPV 450 had a DPP-IV IC50 value of 6.6-0.5 µM. VPV originates from camel beta-CN (f213-215). VPV was reported during the generation of a bovine CN hydrolysate as a potent DPP-IV inhibitor having an IC50 value of 3.19 µM (Yamada, Sakurai & Ochi, 2017). VPV can also be found within other mammalian beta-CNs, including those originating from donkey, chimpanzee, pig and human. VPV is the second most potent DPP-IV inhibitory peptide found in food hydrolysates.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Nongonierma AB, Cadamuro C, Le Gouic A, Mudgil P, Maqsood S, FitzGerald RJ. Dipeptidyl peptidase IV (DPP-IV) inhibitory properties of a camel whey protein enriched hydrolysate preparation. Food Chem. 2019

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.