VPM-p15 peptide

VPM-p15 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

VSXGILLDLSRTSLP, X is 4-methylphenylalanine

H-Val-Ser-Phe(4-Me)-Gly-Ile-Leu-Leu-Asp-Leu-Ser-Arg-Thr-Ser-Leu-Pro-OH

15

95.25%

C₇₅H₁₂₆N₁₈O₂₂

1631.91

Synthetic

Powder

VPM-p15 is an optimised version of the peptide p15, itself an activator derived from the ADGRG2 Stachel sequence, an agonist sequence found within within the large ectodomains of members of the adhesion G protein-coupled receptor (aGPCR) family. VPM-p15 activates ADGRG2 with significantly improved (>2 orders of magnitude) affinity compared to p15. VPM-p15 is a useful tool to investigate the function of ADGRG2, which is a critical regulator of male fertility that maintains ion/pH homeostasis and CFTR coupling.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Sun et al (2021) Optimization of a peptide ligand for the adhesion GPCR ADGRG2 provides a potent tool to explore receptor biology. J Biol Chem. 296 100174 PMID: 33303626

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.