VPM peptide

VPM peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-GCRDVPMSMRGGDRCG-OH

H-Gly-Cys-Arg-Asp-Val-Pro-Met-Ser-Met-Arg-Gly-Gly-Asp-Arg-Cys-Gly-OH

16

95.91%

C₆₃H₁₀₉N₂₅O₂₂S₄

1696.95

Synthetic

Powder

VPM peptide, GCRDVPMSMRGGDRCG, a cysteine-contained biodegradable matrix metalloproteinase (MMP) crosslinker peptide is an enzyme cleavable sequence and can be incorporated into hydrogel backbones to give synthetic hydrogels the capacity to be proteolytically degraded by cell-secreted enzymes. The peptide can be used to study cell migration and matrix remodeling.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• DiMartini ET, Kyker-Snowman K, Shreiber DI. A click chemistry-based, free radical-initiated delivery system for the capture and release of payloads. Drug Deliv. 2023 Dec;30(1):2232952. doi: 10.1080/10717544.2023.2232952. PMID: 37434450; PMCID: PMC10339776.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.