VMAPRTLFL peptide

VMAPRTLFL peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-VMAPRTLFL-OH

H-Val-Met-Ala-Pro-Arg-Thr-Leu-Phe-Leu-OH

9

95.92%

C₄₉H₈₂N₁₂O₁₁S

1047.31

Synthetic

Powder

VMAPRTLFL, a peptide derived from the leader sequence of HLA-G. Adaptive NK cells discriminate HLA-E-bound peptides with exquisite specificity. The HLA-E peptide ligand VMAPRTLFL enriches FcεRg– adaptive NK cells. VMAPRTLFL specifically enhances antibody-driven adaptive NK cell responses. Exposure to VMAPRTLFL leaves other NK cell activation pathways unaffected.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Rölle A, Meyer M, Calderazzo S, Jäger D, Momburg F. Distinct HLA-E Peptide Complexes Modify Antibody-Driven Effector Functions of Adaptive NK Cells. Cell Rep. 2018 Aug 21;24(8):1967-1976.e4. doi: 10.1016/j.celrep.2018.07.069. PMID: 30134159.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.