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Product Name
USP34 antibody
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Description
USP34 Rabbit Polyclonal antibody. Positive IHC detected in human breast cancer tissue, human prostate cancer tissue. Positive IP detected in mouse brain tissue. Positive WB detected in human brain tissue, COLO 320 cells. Observed molecular weight by Western-blot: 47 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
Deubiquitinating enzyme 34 antibody; FLJ43910 antibody; KIAA0570 antibody; KIAA0729 antibody; Ubiquitin thiolesterase 34 antibody; USP34 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: BC022783). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:116608(USP34 antibody) at dilution of 1:600
IP Result of anti-USP34 (IP:Catalog No:116608, 4ug; Detection:Catalog No:116608 1:500) with mouse brain tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:116608(USP34 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:116608(USP34 Antibody) at dilution of 1:50 (under 40x lens)
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Background
USP34, also named as KIAA0570 and KIAA0729, belongs to the peptidase C19 family. USP34 recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. It is involved in the processing of poly-ubiquitin precursors as well as that of ubiquinated proteins. The antibody recognize all these 3 isoform of USP34.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"