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Product Name
USP3 antibody
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Description
USP3 Rabbit Polyclonal antibody. Positive IF detected in MCF-7 cells. Positive IHC detected in human pancreas tissue. Positive IP detected in mouse liver tissue. Positive WB detected in K-562 cells, RAW264.7 cells. Observed molecular weight by Western-blot: 53-59 kDa
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Tested applications
ELISA, IHC, IF, WB, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
Deubiquitinating enzyme 3 antibody; SIH003 antibody; ubiquitin specific peptidase 3 antibody; Ubiquitin thiolesterase 3 antibody; UBP antibody; USP3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of USP3 recombinant protein (Accession Number: NM_006537). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:116604(USP3 antibody) at dilution of 1:500
IP Result of anti-USP3 (IP:Catalog No:116604, 4ug; Detection:Catalog No:116604 1:500) with mouse liver tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human pancreas tissue slide using Catalog No:116604(USP3 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human pancreas tissue slide using Catalog No:116604(USP3 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of MCF-7 cells using Catalog No:116604(USP3 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
USP3(Ubiquitin carboxyl-terminal hydrolase 3) belongs to the peptidase C19 family. It may regulate the DNA damage response (DDR) checkpoint through deubiquitination of H2A at DNA damage sites. USP3 is implicated as a novel chromatin modifier in the maintenance of genome integrity. This protein has 2 isoforms produced by alternative splicing.
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