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Product Name
USP21 antibody
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Description
USP21 Rabbit Polyclonal antibody. Positive WB detected in RAW 264.7 cells, recombinant ptotein. Positive IHC detected in human heart tissue, human pancreas tissue. Observed molecular weight by Western-blot: 63 kDa
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Tested applications
ELISA, IHC, WB
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Species reactivity
Human; other species not tested.
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Alternative names
Deubiquitinating enzyme 21 antibody; Ubiquitin thiolesterase 21 antibody; USP16 antibody; USP21 antibody; USP23 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of USP21 recombinant protein (Accession Number: NM_012475). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
RAW 264.7 cells were subjected to SDS PAGE followed by western blot with Catalog No:116599(USP21 Antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:116599(USP21 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:116599(USP21 Antibody) at dilution of 1:50 (under 40x lens)
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Background
USP21(Ubiquitin carboxyl-terminal hydrolase 21) is also named as USP23.The USPs belong to a subclass of the protein-deubiquitinating enzyme (DUB) superfamily that are categorized into five subclasses based on their ubiquitin-protease domains in the human genome and have been shown to be involved in a broad range of biological activities(PMID:19910467). USP21 is expressed at different levels in the tissues examined. The highest level of USP21 expression is found in the heart, pancreas, and skeletal muscle. USP21 messages could also be detected in the brain, placenta, liver, and kidney but are very low in the Lung through the northern blot(PMID:10799498).
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