USP21 antibody

Cat.#: 116599

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Product Information

  • Product Name
    USP21 antibody
  • Documents
  • Description
    USP21 Rabbit Polyclonal antibody. Positive WB detected in RAW 264.7 cells, recombinant ptotein. Positive IHC detected in human heart tissue, human pancreas tissue. Observed molecular weight by Western-blot: 63 kDa
  • Tested applications
    ELISA, IHC, WB
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    Deubiquitinating enzyme 21 antibody; Ubiquitin thiolesterase 21 antibody; USP16 antibody; USP21 antibody; USP23 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of USP21 recombinant protein (Accession Number: NM_012475). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IHC: 1:20-1:200

  • Validations

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with Catalog No:116599(USP21 Antibody) at dilution of 1:600

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with Catalog No:116599(USP21 Antibody) at dilution of 1:600

    Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:116599(USP21 Antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:116599(USP21 Antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:116599(USP21 Antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:116599(USP21 Antibody) at dilution of 1:50 (under 40x lens)

  • Background
    USP21(Ubiquitin carboxyl-terminal hydrolase 21) is also named as USP23.The USPs belong to a subclass of the protein-deubiquitinating enzyme (DUB) superfamily that are categorized into five subclasses based on their ubiquitin-protease domains in the human genome and have been shown to be involved in a broad range of biological activities(PMID:19910467). USP21 is expressed at different levels in the tissues examined. The highest level of USP21 expression is found in the heart, pancreas, and skeletal muscle. USP21 messages could also be detected in the brain, placenta, liver, and kidney but are very low in the Lung through the northern blot(PMID:10799498).

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