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Product Name
USP2 antibody
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Description
USP2 Rabbit Polyclonal antibody. Positive WB detected in mouse brain tissue, MCF7 cells, mouse kidney tissue, rat brain tissue. Positive IHC detected in human breast cancer tissue, human prostate cancer tissue, human skeletal muscle tissue. Observed molecular weight by Western-blot: 69kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
Deubiquitinating enzyme 2 antibody; ubiquitin specific peptidase 2 antibody; Ubiquitin thiolesterase 2 antibody; UBP41 antibody; USP2 antibody; USP9 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of USP2 recombinant protein (Accession Number: XM_005271721). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:116596(USP2 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:116596(USP2 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:116596(USP2 antibody) at dilution of 1:50 (under 40x lens)
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Background
Ubiquitin, a highly conserved protein involved in the regulation of intracellular protein breakdown, cell cycle regulation, and stress response, is released from degraded proteins by disassembly of the polyubiquitin chains. The disassembly process is mediated by ubiquitin-specific proteases (USPs). USP2 is also named as UBP41. USP2 can interact indirectly with multiple clock proteins, so it remains possible that USP2 may modulate the ubiquitination and function of these other proteins. It has 4 isoforms produced by alternative splicing. This antibody is specific to the isoform 1(USP2a) of USP2.
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References
- Wang S, Wu H, Liu Y. Expression of USP2-69 in mesangial cells in vivo and in vitro. Pathology international. 60(3):184-92. 2010.
- Kitamura H, Kimura S, Shimamoto Y. Ubiquitin-specific protease 2-69 in macrophages potentially modulates metainflammation. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 27(12):4940-53. 2013.
- Qu Q, Mao Y, Xiao G. USP2 promotes cell migration and invasion in triple negative breast cancer cell lines. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 36(7):5415-23. 2015.
- Kwon DN, Choi YJ, Cho SG. CMP-Neu5Ac Hydroxylase Null Mice as a Model for Studying Metabolic Disorders Caused by the Evolutionary Loss of Neu5Gc in Humans. BioMed research international. 2015:830315. 2015.
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