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Product Name
USP11 antibody
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Description
USP11 Rabbit Polyclonal antibody. Positive IHC detected in human hepat℃irrhosis tissue, human brain tissue, human testis tissue. Positive IF detected in HEK-293 cells. Positive WB detected in A549 cells, HEK-293 cells, human testis tissue, Jurkat cells. Positive IP detected in HEK-293 cells. Observed molecular weight by Western-blot: 110 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human; other species not tested.
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Alternative names
Deubiquitinating enzyme 11 antibody; Ubiquitin thiolesterase 11 antibody; UHX1 antibody; USP11 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of USP11 recombinant protein (Accession Number: NM_001371072). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:1000-1:10000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
A549 cells were subjected to SDS PAGE followed by western blot with Catalog No:116587(USP11 antibody) at dilution of 1:1000
IP Result of anti-USP11 (IP:Catalog No:116587, 4ug; Detection:Catalog No:116587 1:2000) with HEK-293 cells lysate 2400ug.
Immunohistochemistry of paraffin-embedded human hepatocirrhosis tissue slide using Catalog No:116587(USP11 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human hepatocirrhosis tissue slide using Catalog No:116587(USP11 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HEK-293 cells using Catalog No:116587(USP11 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
USP11(Ubiquitin carboxyl-terminal hydrolase 11) is also named as UHX1 and belongs to the peptidase C19 family. It is a deubiquitinating enzyme that controls many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction.
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