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Product Name
UROS antibody
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Description
UROS Rabbit Polyclonal antibody. Positive IHC detected in human cervical cancer tissue. Positive IF detected in MCF-7 cells. Positive IP detected in K-562 cells. Positive WB detected in K-562 cells. Observed molecular weight by Western-blot: 29.5 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
UROIIIS antibody; uroporphyrinogen III synthase antibody; UROS antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of UROS recombinant protein (Accession Number: NM_000375). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:116578(UROS antibody) at dilution of 1:600
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:116578(UROS antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:116578(UROS antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of MCF-7 cells, using UROS antibody Catalog No:116578 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-UROS (IP:Catalog No:116578, 4ug; Detection:Catalog No:116578 1:500) with K-562 cells lysate 4000ug.
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Background
UROS represents uroporphyrinogen III synthase
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