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Product Name
UQCRQ antibody
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Description
UQCRQ Rabbit Polyclonal antibody. Positive IHC detected in human liver cancer tissue. Positive IF detected in HepG2 cells. Positive WB detected in human liver tissue, HepG2 cells. Observed molecular weight by Western-blot: 10 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Complex III subunit 8 antibody; Complex III subunit VIII antibody; QCR8 antibody; QP C antibody; QPC antibody; UQCRQ antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of UQCRQ recombinant protein (Accession Number: NM_014402). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
human liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:116639(UQCRQ antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:116639(UQCRQ antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:116639(UQCRQ antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:116639(UQCRQ Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
UQCRQ(Cytochrome b-c1 complex subunit 8) belongs to the UQCRQ/QCR8 family. UQCRQ is a ubiquinone-binding protein localized to the cytochrome bc1 region of the mitochondrial respiratory chain. The deduced 110-amino acid protein has a relatively hydrophobic and basic N-terminal region, an aspartic acid-rich middle region, and a glutamic acid- and lysine-rich C-terminal region.
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References
- Jain M, Rivera S, Monclus EA. Mitochondrial reactive oxygen species regulate transforming growth factor-β signaling. The Journal of biological chemistry. 288(2):770-7. 2013.
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