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Product Name
UQCRC1 antibody
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Description
UQCRC1 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in human brain tissue, HeLa cells, HepG2 cells, human heart tissue, human skeletal muscle tissue, L02 cells, MCF7 cells, mouse brain tissue, mouse kidney tissue, mouse large intestine tissue, mouse skeletal muscle tissue, mouse spleen tissue. Positive IF detected in HepG2 cells. Positive IHC detected in human heart tissue, human liver tissue. Observed molecular weight by Western-blot: 45kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Complex III subunit 1 antibody; Core protein I antibody; D3S3191 antibody; QCR1 antibody; UQCR1 antibody; UQCRC1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of UQCRC1 recombinant protein (Accession Number: NM_003365). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:116635(UQCRC1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human heart using Catalog No:116635(UQCRC1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human heart using Catalog No:116635(UQCRC1 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using UQCRC1 antibody Catalog No:116635 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-UQCRC1 (IP:Catalog No:116635, 4ug; Detection:Catalog No:116635 1:500) with HeLa cells lysate 1600ug.
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Background
UQCRC1(Cytochrome b-c1 complex subunit 1, mitochondrial), also named as QCR1 and UQCR1, is a ubunit of the cytochrome bc1 complex (complex III) of the mitochondrial respiratory chain, may mediate the formation of the complex between cytochromes c and c1. The gene encodes a 53 kDa protein with a 34 amino acids transit peptide. It is associated with apoptosis or inhibition of cancer cell growth.
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References
- Liu CI, Chen CC, Chen JC. Proteomic analysis of anti-tumor effects of 11-dehydrosinulariolide on CAL-27 cells. Marine drugs. 9(7):1254-72. 2011.
- Li HH, Su JH, Chiu CC. Proteomic investigation of the sinulariolide-treated melanoma cells A375: effects on the cell apoptosis through mitochondrial-related pathway and activation of caspase cascade. Marine drugs. 11(7):2625-42. 2013.
- Xu Q, Xia P, Li X, Wang W, Liu Z, Gao X. Tetramethylpyrazine ameliorates high glucose-induced endothelial dysfunction by increasing mitochondrial biogenesis. PloS one. 9(2):e88243. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"