UGCG Rabbit Polyclonal antibody. Positive IP detected in A375 cells. Positive WB detected in A375 cells, mouse brain tissue. Positive IHC detected in human lymphoma tissue. Observed molecular weight by Western-blot: 50-55 kDa
ELISA, WB, IHC, IP
Human,Mouse,Rat; other species not tested.
Ceramide glucosyltransferase antibody; GCS antibody; GLCT 1 antibody; Glucosylceramide synthase antibody; UGCG antibody
This antibody was obtained by immunization of UGCG recombinant protein (Accession Number: BC038711). Purification method: Antigen affinity purified.
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Store at -20℃. DO NOT ALIQUOT
A375 cells were subjected to SDS PAGE followed by western blot with Catalog No:116553(UGCG antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:116553(UGCG antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-UGCG (IP:Catalog No:116553, 4ug; Detection:Catalog No:116553 1:500) with A375 cells lysate 3200ug.
UGCG, also named as GCS and GLCT-1, belongs to the glycosyltransferase 2 family. It catalyzes the first glycosylation step in glycosphingolipid biosynthesis, the transfer of glucose to ceramide. UGCG may also serve as a "flippase". It is up-regulated at the transcriptional level during keratinocyte differentiation. UGCG is a membrane protein. The calculated MW is 45kd. In SDS-PAGE, it is about 50-55kd.
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