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  • UGCG antibody
  • UGCG antibody
  • UGCG antibody

UGCG antibody

Cat.#: 116553

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Special Price 239.0 USD

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Product Information

  • Product Name
    UGCG antibody
  • Documents
  • Description
    UGCG Rabbit Polyclonal antibody. Positive IP detected in A375 cells. Positive WB detected in A375 cells, mouse brain tissue. Positive IHC detected in human lymphoma tissue. Observed molecular weight by Western-blot: 50-55 kDa
  • Tested applications
    ELISA, WB, IHC, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    Ceramide glucosyltransferase antibody; GCS antibody; GLCT 1 antibody; Glucosylceramide synthase antibody; UGCG antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of UGCG recombinant protein (Accession Number: BC038711). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

  • Validations

    A375 cells were subjected to SDS PAGE followed by western blot with Catalog No:116553(UGCG antibody) at dilution of 1:1000

    A375 cells were subjected to SDS PAGE followed by western blot with Catalog No:116553(UGCG antibody) at dilution of 1:1000

    Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:116553(UGCG antibody) at dilution of 1:100 (under 40x lens)

    Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:116553(UGCG antibody) at dilution of 1:100 (under 40x lens)

    IP Result of anti-UGCG (IP:Catalog No:116553, 4ug; Detection:Catalog No:116553 1:500) with A375 cells lysate 3200ug.

    IP Result of anti-UGCG (IP:Catalog No:116553, 4ug; Detection:Catalog No:116553 1:500) with A375 cells lysate 3200ug.

  • Background
    UGCG, also named as GCS and GLCT-1, belongs to the glycosyltransferase 2 family. It catalyzes the first glycosylation step in glycosphingolipid biosynthesis, the transfer of glucose to ceramide. UGCG may also serve as a "flippase". It is up-regulated at the transcriptional level during keratinocyte differentiation. UGCG is a membrane protein. The calculated MW is 45kd. In SDS-PAGE, it is about 50-55kd.
  • References
  • cDNA Clones

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"