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Product Name
UGCG antibody
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Description
UGCG Rabbit Polyclonal antibody. Positive IP detected in A375 cells. Positive WB detected in A375 cells, mouse brain tissue. Positive IHC detected in human lymphoma tissue. Observed molecular weight by Western-blot: 50-55 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Ceramide glucosyltransferase antibody; GCS antibody; GLCT 1 antibody; Glucosylceramide synthase antibody; UGCG antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of UGCG recombinant protein (Accession Number: NM_003358). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
A375 cells were subjected to SDS PAGE followed by western blot with Catalog No:116553(UGCG antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:116553(UGCG antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-UGCG (IP:Catalog No:116553, 4ug; Detection:Catalog No:116553 1:500) with A375 cells lysate 3200ug.
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Background
UGCG, also named as GCS and GLCT-1, belongs to the glycosyltransferase 2 family. It catalyzes the first glycosylation step in glycosphingolipid biosynthesis, the transfer of glucose to ceramide. UGCG may also serve as a "flippase". It is up-regulated at the transcriptional level during keratinocyte differentiation. UGCG is a membrane protein. The calculated MW is 45kd. In SDS-PAGE, it is about 50-55kd.
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