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Product Name
UCP1 antibody
- Documents
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Description
UCP1 Rabbit Polyclonal antibody. Positive WB detected in mouse brown adipose tissue. Positive IHC detected in rat brain tissue. Observed molecular weight by Western-blot: 30-33 kDa
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Tested applications
ELISA, IHC, WB
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
SLC25A7 antibody; Thermogenin antibody; UCP antibody; UCP 1 antibody; UCP1 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of UCP1 recombinant protein (Accession Number: NM_021833). Purification method: Antigen Affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse brown adipose tissue were subjected to SDS PAGE followed by western blot with Catalog No:116545(UCP1 antibody) at dilution of 1:600
Immunohistochemical of paraffin-embedded rat brain using Catalog No:116545(UCP1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded rat brain using Catalog No:116545(UCP1 antibody) at dilution of 1:50 (under 40x lens)
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Background
UCP-1 (Mitochondrial uncoupling protein 1), is a mitochondrial transporter protein that creates proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. It has been identified a key molecule for metabolic thermogenesis to avoid an excess of fat accumulation. UCP-1 expression is usually restricted to brown adipose when induced by cold exposure and thyroid hormone.
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References
- Wang Q, Dong Z, Liu X. Programmed cell death-4 deficiency prevents diet-induced obesity, adipose tissue inflammation, and insulin resistance. Diabetes. 62(12):4132-43. 2013.
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