Human UBE1 / UBA1 (His & GST Tag) recombinant protein

Human UBE1 / UBA1 (His & GST Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

UBE1, also known as UBA1, belongs to the ubiquitin-activating E1 family. UBE1 gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. UBE1 catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. It also catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP. Defects in UBA1 can cause spinal muscular atrophy X-linked type 2 (SMAX2), also known as X-linked lethal infantile spinal muscular atrophy, distal X-linked arthrogryposis multiplex congenita or X-linked arthrogryposis type 1 (AMCX1). Spinal muscular atrophy refers to a group of neuromuscular disorders characterized by degeneration of the anterior horn cells of the spinal cord, leading to symmetrical muscle weakness and atrophy. SMAX2 is a lethal infantile form presenting with hypotonia, areflexia, and multiple congenital contractures.

Ubiquitin-activating enzyme E1 (A1S9T and BN75 temperature sensitivity complementing), isoform CRA_a

GXP1; UBE1; UBA1; POC20 CENTRIOLAR PROTEIN HOMOLOG; UBIQUITIN-LIKE MODIFIER-ACTIVATING ENZYME 1 X; A1ST; A1S9T AND BN75 TEMPERATURE SENSITIVITY COMPLEMENTING; UBIQUITIN-LIKE MODIFIER-ACTIVATING ENZYME 1; RP23-394F6.2; UBIQUITIN-ACTIVATING ENZYME E1; UBIQUITIN-ACTIVATING ENZYME E1 X; SMAX2; CTD-2522E6.1; A1S9T; POC20; SBX; UBE-1; AA989744; CHR X; AMCX1; UBIQUITIN-ACTIVATING ENZYME E1 HOMOLOG A; UBE1X; A1S9; MGC4781; UBA1A

P22314

UBE1; hCG_17406

Baculovirus-Insect Cells

A DNA sequence encoding the human UBA1 (NP_003325.2) (Ser 2-Arg 1058) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Human

The recombinant human UBA1/GST chimera consists of 1294 amino acids and has a calculated molecular mass of 146 KDa. It migrates as an approximately 130 KDa band in SDS-PAGE under reducing conditions.

> 96 % as determined by SDS-PAGE