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Product Name
UBC9 antibody
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Description
UBC9 Rabbit Polyclonal antibody. Positive IHC detected in human lung cancer tissue. Positive WB detected in mouse testis tissue, HEK-293 cells, mouse kidney tissue, mouse thymus tissue. Observed molecular weight by Western-blot: 18kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
C358B7.1 antibody; UBE2I antibody; P18 antibody; SUMO conjugating enzyme UBC9 antibody; SUMO protein ligase antibody; UBC9 antibody; UBCE9 antibody; UBE2I antibody; Ubiquitin carrier protein 9 antibody; Ubiquitin carrier protein I antibody; Ubiquitin protein ligase I antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of UBC9 recombinant protein (Accession Number: BC051289). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:116508(UBC9 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:116508(UBC9 antibody) at dilution of 1:50 (under 10x lens)
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Background
UBE2I is also named as UBC9, UBCE9 and belongs to the ubiquitin-conjugating enzyme family. It is a homologue of the E2 ubiquitin conjugating enzyme and participates in the covalent linking of SUMO-1 molecule to the target protein. This protein is present at a high level in spleen and lung. Moderate level of UBE2I is detected in kidney and liver. Low amount of UBE2I is observed in brain, whereas the 18 kDa band of UBE2I is barely visible or absent in heart and skeletal muscle. In heart and muscle extracts the UBE2I antibodies recognizes a 38 kDa protein band,but this band is not visible in extracts of other rat tissues(PMID:14739995).
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References
- Zhong N, Radu G, Ju W, Brown WT. Novel progerin-interactive partner proteins hnRNP E1, EGF, Mel 18, and UBC9 interact with lamin A/C. Biochemical and biophysical research communications. 338(2):855-61. 2005.
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