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Product Name
UBASH3A-Specific antibody
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Description
UBASH3A-Specific Rabbit Polyclonal antibody. Positive WB detected in human spleen tissue. Positive IP detected in mouse spleen tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 69 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Cbl interacting protein 4 antibody; CLIP4 antibody; STS 2 antibody; STS2 antibody; T cell ubiquitin ligand antibody; TULA antibody; UBASH3A antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_018961). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IF: 1:10-1:100
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Validations
human spleen tissue were subjected to SDS PAGE followed by western blot with Catalog No:116474(UBASH3A-Specific antibody) at dilution of 1:400
Immunofluorescent analysis of HepG2 cells, using UBASH3A antibody Catalog No:116474 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-UBASH3A-Specific (IP:Catalog No:116474, 4ug; Detection:Catalog No:116474 1:500) with mouse spleen tissue lysate 4000ug.
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Background
UBASH3A gene encodes ubiquitin-associated and SH3 domain-containing protein A (UBASH3A) also known as T-cell ubiquitin ligand 1 (TULA1) which is one of two family members belonging to the T-cell ubiquitin ligand (TULA) family. UBASH3A gene expression is limited to only a few tissues, with its highest expression in spleen, peripheral blood leukocytes, and bone marrow. UBASH3A protein is associated with c-Cbl and ubiquitylated proteins and has been implicated in the regulation of signaling mediated by protein-tyrosine kinases oth family members can negatively regulate T-cell signaling. UBASH3A can facilitate growth factor withdrawal-induced apoptosis in T cells via its interaction with apoptosis-inducing factor (AIF). This antibody is UBASH3A-specific.
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