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Product Name
UBAP1 antibody
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Description
UBAP1 Rabbit Polyclonal antibody. Positive IF detected in Hela cells. Positive IHC detected in human lung cancer tissue, human heart tissue, human lung tissue. Positive IP detected in mouse brain tissue. Positive WB detected in human heart tissue, A431 cells, human lung tissue, human spleen tissue, human testis tissue, mouse brain tissue, mouse lung tissue, mouse testis tissue, mouse thymus tissue, Y79 cells. Observed molecular weight by Western-blot: 55kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
NAG20 antibody; UAP antibody; UBAP antibody; UBAP 1 antibody; UBAP1 antibody; ubiquitin assOCiated protein 1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of UBAP1 recombinant protein (Accession Number: NM_001171203). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
human heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:116473(UBAP1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:116473(UBAP1 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of Hela cells, using UBAP1 antibody Catalog No:116473 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-UBAP1 (IP:Catalog No:116473, 4ug; Detection:Catalog No:116473 1:500) with mouse brain tissue lysate 4000ug.
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Background
UBAP1, also named as NAG20, is a 55 kDa protein which contains 2 putative tandem UBA domains at the C terminus, a coiled-coil domain, 2 possible N-glycosylation sites, 5 PKC phosphorylation sites, 7 casein kinase II phosphorylation sites, and 3 N-myristoylation sites. UBAP1 might be a potential effective diagnosis candidate for NPC and decreased expression of UBAP1 protein is a possible point of dysfunction along the pathogenesis pathway for NPC that may contribute to malignant transformation.
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References
- Verma R, Marchese A. The endosomal sorting complex required for transport pathway mediates chemokine receptor CXCR4-promoted lysosomal degradation of the mammalian target of rapamycin antagonist DEPTOR. The Journal of biological chemistry. 290(11):6810-24. 2015.
- Kueck T, Foster TL, Weinelt J, Sumner JC, Pickering S, Neil SJ. Serine Phosphorylation of HIV-1 Vpu and Its Binding to Tetherin Regulates Interaction with Clathrin Adaptors. PLoS pathogens. 11(8):e1005141. 2015.
- Kharitidi D, Apaja PM, Manteghi S. Interplay of Endosomal pH and Ligand Occupancy in Integrin α5β1 Ubiquitination, Endocytic Sorting, and Cell Migration. Cell reports. 13(3):599-609. 2015.
- Agromayor M, Soler N, Caballe A. The UBAP1 subunit of ESCRT-I interacts with ubiquitin via a SOUBA domain. Structure (London, England : 1993). 20(3):414-28. 2012.
- Kueck T, Neil SJ. A cytoplasmic tail determinant in HIV-1 Vpu mediates targeting of tetherin for endosomal degradation and counteracts interferon-induced restriction. PLoS pathogens. 8(3):e1002609. 2012.
- Stefani F, Zhang L, Taylor S. UBAP1 is a component of an endosome-specific ESCRT-I complex that is essential for MVB sorting. Current biology : CB. 21(14):1245-50. 2011.
- Zhang L, Deng T, Li X. microRNA-141 is involved in a nasopharyngeal carcinoma-related genes network. Carcinogenesis. 31(4):559-66. 2010.
- Wunderley L, Brownhill K, Stefani F, Tabernero L, Woodman P. The molecular basis for selective assembly of the UBAP1-containing endosome-specific ESCRT-I complex. Journal of cell science. 127(Pt 3):663-72. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"