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Product Name
UBA3 Polyclonal Antibody
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Description
Polyclonal antibody to UBA3
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Tested applications
IF
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Species reactivity
Human
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Alternative names
UBA3 antibody; NAE2 antibody; UBE1C antibody; hUBA3 antibody; ubiquitin like modifier activating enzyme 3 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 234-463 of human UBA3 (NP_003959.3).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
IF 1:50 - 1:200
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Validations
Immunofluorescence - UBA3 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using UBA3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"