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Product Name
Human PTP1B / PTPN1 (His Tag) recombinant protein
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Description
PTP1B, also known as PTPN1, belongs to the protein-tyrosine phosphatase (PTP) family. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTP1B contains 1 tyrosine-protein phosphatase domain and is expressed in many tissues. PTP1B is localized to the cytoplasmic face of the endoplasmic reticulum. PTP1B was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of PTP1B in cell growth control, and cell response to IFN stimulation.
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Protein name
Tyrosine-protein phosphatase non-receptor type
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Protein short names
PTPN1; PTP-1B; RP23-58A8.1; PTP-HA2; PTP1B
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Uniprot ID
P18031
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Gene Name
PTPN1; hCG_37134
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
A DNA sequence encoding the human PTPN1 (NP_002818.1) (Glu 2-Asn 321) was expressed, with a polyhistide tag at the N-terminus.
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Protein Species
Human
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Molecular weight
The recombinant human PTPN1consisting of 327 amino acids and has a calculated molecular mass of 38 kDa.
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Purity
> 95 % as determined by SDS-PAGE
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Activity
Measured by its ability to dephosphorylate a phosphotyrosine residue in an EGF receptor (aa988-998) phosphopeptide substrate, R&D Systems, Catalog # ES006.
The specific activity is > 15 nmoles/min/μg. -
Validations
Human PTP1B / PTPN1 Protein (His Tag)
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