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TTRAP antibody

Cat.#: 116482

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Product Information

  • Product Name
    TTRAP antibody
  • Documents
  • Description
    TTRAP Rabbit Polyclonal antibody. Positive WB detected in human spleen tissue. Positive IP detected in mouse spleen tissue. Observed molecular weight by Western-blot: 49kd
  • Tested applications
    ELISA, WB, IP
  • Species reactivity
    Human, Mouse, Rat; other species not tested.
  • Alternative names
    5 Tyr DNA phosphodiesterase antibody; AD022 antibody; dJ30M3.3 antibody; EAP2 antibody; EAPII antibody; ETS1 assOCiated protein 2 antibody; ETS1 assOCiated protein II antibody; TDP2 antibody; TTRAP antibody; Tyr DNA phosphodiesterase 2 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of TTRAP recombinant protein (Accession Number: NM_016614). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

  • Validations

    human spleen tissue were subjected to SDS PAGE followed by western blot with Catalog No:116482(TTRAP antibody) at dilution of 1:500

    human spleen tissue were subjected to SDS PAGE followed by western blot with Catalog No:116482(TTRAP antibody) at dilution of 1:500

    IP Result of anti-TTRAP (IP:Catalog No:116482, 4ug; Detection:Catalog No:116482 1:500) with mouse spleen tissue lysate 4800ug.

    IP Result of anti-TTRAP (IP:Catalog No:116482, 4ug; Detection:Catalog No:116482 1:500) with mouse spleen tissue lysate 4800ug.

  • Background
    TTRAP is a 5-prime tyrosyl-DNA phosphodiesterase that is required for efficient repair of topoisomerase II-induced DNA double-strand breaks. Thus it can remove lots of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, generating a free 5' phosphate to DNA. It also has 3'-tyrosyl DNA phophodiesterase activity, but not so efficient as TDP1. TTRAP functions as a negative regulator of ETS1 and inhibit nuclear factor-kappa-B activation.
  • References
    • Thomson G, Watson A, Caldecott K. Generation of assays and antibodies to facilitate the study of human 5'-tyrosyl DNA phosphodiesterase. Analytical biochemistry. 436(2):145-50. 2013.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"