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Product Name
TSPAN9 antibody
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Description
TSPAN9 Rabbit Polyclonal antibody. Positive WB detected in mouse peripheral blood leuk℃yte tissue, mouse spleen tissue, rat spleen tissue. Positive IP detected in mouse spleen tissue. Observed molecular weight by Western-blot: 29-33 kDa
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Tested applications
ELISA, IP, WB
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
NET 5 antibody; NET5 antibody; PP1057 antibody; Tetraspan NET 5 antibody; tetraspanin 9 antibody; Tspan 9 antibody; TSPAN9 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TSPAN9 recombinant protein (Accession Number: XM_047428126). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:2000
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Validations
mouse peripheral blood leukocyte tissue were subjected to SDS PAGE followed by western blot with Catalog No:116366(TSPAN9 Antibody) at dilution of 1:300
IP Result of anti-TSPAN9 (IP:Catalog No:116366, 4ug; Detection:Catalog No:116366 1:500) with mouse spleen tissue lysate 4800ug.
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Background
TSPAN9 is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Tetraspanins have been involved in diverse processes such as cell activation and proliferation, adhesion and motility, differentiation, and cancer. TSPAN9 is relatively highly expressed in the megakaryocyte/platelet lineage and has a role in regulating platelet function in concert with other platelet tetraspanins and their associated proteins. TSPAN9 has been identified as a glycoprotein (PMID: 18795891). This polyclonal antibody detects endogenous TSPAN9 at a band of 29-33 kDa, which is larger than the calculated molecular weight of TSPAN9 is 27 kDa, probably due to glycosylation.
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