-
Product Name
TSPAN12 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to TSPAN12
-
Tested applications
WB, IHC, FC
-
Species reactivity
Human
-
Alternative names
TSPAN12 antibody; EVR5 antibody; NET-2 antibody; NET2 antibody; TM4SF12 antibody; tetraspanin-12 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: A synthetic Peptide of human TSPAN12
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, pH7.3.
-
Storage instructions
Store at 4℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:1000
IHC 1:20 - 1:50
FC 1:20 - 1:50 -
Validations
Western blot - TSPAN12 Polyclonal Antibody
Western blot analysis of extracts of T47D cells, using TSPAN12 antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
-
Background
Regulator of cell surface receptor signal transduction. Plays a central role in retinal vascularization by regulating norrin (NDP) signal transduction. Acts in concert with norrin (NDP) to promote FZD4 multimerization and subsequent activation of FZD4, leading to promote accumulation of beta-catenin (CTNNB1) and stimulate LEF/TCF-mediated transcriptional programs. Suprisingly, it only activate the norrin (NDP)-dependent activation of FZD4, while it does not activate the Wnt-dependent activation of FZD4, suggesting the existence of a Wnt-independent signaling that also promote accumulation the beta-catenin (CTNNB1) (By similarity). Acts as a regulator of membrane proteinases such as ADAM10 and MMP14/MT1-MMP. Activates ADAM10-dependent cleavage activity of amyloid precursor protein (APP). Activates MMP14/MT1-MMP-dependent cleavage activity.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"