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Product Name
TSP50 antibody
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Description
TSP50 Rabbit Polyclonal antibody. Positive WB detected in MCF7 cells, HEK-293 cells, human testis tissue, mouse testis tissue. Positive IHC detected in human ovary tumor tissue, human breast cancer tissue, human testis tissue. Positive IF detected in MCF-7 cells. Observed molecular weight by Western-blot: 50-55 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
Cancer/testis antigen 20 antibody; CT20 antibody; PRSS50 antibody; Serine protease 50 antibody; testes protease 50 antibody; TSP50 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TSP50 recombinant protein (Accession Number: NM_013270). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:116439(TSP50 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:116439(TSP50 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of MCF-7 cells using Catalog No:116439(TSP50 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
TSP50 (Testis-specific protease-like protein 50) is also named as PRSS50, CT20 and belongs to the peptidase S1 family. It can enhance the expression of NFKB target genes involved in cell proliferation, and promotes cell proliferation, at least partially, through activation of the NFKB signalling pathway(PMID:21385156). There are two protein bands of 37 kDa and 43 kDa to be detected in human testes that the the difference is most likely caused by posttranslational proteolytic cleavage(PMID:11782390). The full length protein has a signal peptide with 39 amino acids and two glycosylation sites.
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References
- Liu F, Cao Q, Liu N. Overexpression of Testes-Specific Protease 50 (TSP50) Predicts Poor Prognosis in Patients with Gastric Cancer. Gastroenterology research and practice. 2014:498246. 2014.
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