TSHR antibody

TSHR antibody

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TSHR Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, mouse ovary tissue, mouse thymus tissue. Observed molecular weight by Western-blot: 62-70kd

ELISA, WB

Human, Mouse; other species not tested.

CHNG1 antibody; hTSHR I antibody; LGR3 antibody; Thyrotropin receptor antibody; TSH R antibody; TSHR antibody

Rabbit IgG

This antibody was obtained by immunization of TSHR recombinant protein (Accession Number: NM_001018036). Purification method: Antigen affinity purified.

Polyclonal

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Store at -20℃. DO NOT ALIQUOT

Thyroid stimulating hormone (also known as thyrotropin or TSH) is a glycoprotein hormone produced by thyrotrophs in the anterior pituitary gland. The TSH receptor (TSHR), mainly expressed on the thyroid epithelial cells, is a G protein-coupled receptor (GPCR) of the seven-transmembrane domain family and plays a central role in controlling thyroid cell metabolism. The gene of TSHR maps to chromosome 14q31.1, and encodes a 764-amino acid protein with a predicted unmodified molecular weight of 86.8 kDa. The apparent molecular weight of glycosylated form is 95-120 kDa. Studies reveal that TSHR is composed of two subunits, A and B, which are produced by cleavage of single-chain TSHR on the cell surface and subsequently connected by disulfide bonds. This rabbit polyclonal antibody raised against N-terminal region (1-253 aa) of human TSHR can detect subunit A with an experimentally determined molecular weight of 62-70 kDa under reducing condition. (PMID: 20578897, 17158770, 12593718)

• Endo T, Kobayashi T. Dominant negative effect of mutated thyroid stimulating hormone receptor (P556L) causes hypothyroidism in C.RF-Tshr(hyt/wild) mice. PloS one. 7(8):e42358. 2012.