Product Name

TSC22D1 antibody

Documents

Description

TSC22D1 Rabbit Polyclonal antibody. Positive WB detected in human brain tissue, A549 cells, rat brain tissue. Positive IP detected in rat brain tissue. Positive IHC detected in human brain tissue, human gliomas tissue, human testis tissue. Observed molecular weight by Western-blot: 16 kDa

Tested applications

ELISA, WB, IHC, IP

Species reactivity

Human, Rat, Mouse; other species not tested.

Alternative names

Cerebral protein 2 antibody; DKFZp686O19206 antibody; KIAA1994 antibody; Regulatory protein TSC 22 antibody; RP11 269C23.2 antibody; TGFB1I4 antibody; TSC22 antibody; TSC22 domain family protein 1 antibody; TSC22 domain family antibody; member 1 antibody; TSC22D1 antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of TSC22D1 recombinant protein (Accession Number: NM_006022). Purification method: Protein A purified.

Clonality

Polyclonal

Formulation

PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:200-1:2000

IP: 1:200-1:2000

IHC: 1:20-1:200

Validations

human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:116423(TSC22D1 antibody) at dilution of 1:1000

IP Result of anti-TSC22D1 (IP:Catalog No:116423, 4ug; Detection:Catalog No:116423 1:500) with rat brain tissue lysate 4000ug.

Immunohistochemistry of paraffin-embedded human brain tissue slide using Catalog No:116423(TSC22D1 Antibody) at dilution of 1:50 (under 40x lens)

Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:116423(TSC22D1 Antibody) at dilution of 1:50 (under 40x lens)

Background

TSC22 domain family 1, transforming growth factor beta 1 induced transcript 4, (TSC22D1, synonyms: TSC22, TGFB1I4, MGC17597) is a member of a family of leucine zipper containing transcription factors with repressor activity and belongs to the large family of early response genes. TSC-22 regulates cell growth and differentiation, and cell death. Leucine zipper structure of TSC22 inhibits the anchorage independent growth of cancer cells. TSC-22 is also an important downstream component of peroxisome proliferator-activated receptor (PPAR) and TGF-beta signaling during intestinal epithelial cell differentiation. This antibody is a rabbit polyclonal antibody raised against an internal region of human TSC22D1.

References

Sprenger CC, Haugk K, Sun S. Transforming Growth Factor-{beta}-Stimulated Clone-22 Is an Androgen-Regulated Gene That Enhances Apoptosis in Prostate Cancer following Insulin-Like Growth Factor-I Receptor Inhibition. Clinical cancer research : an official journal of the American Association for Cancer Research. 15(24):7634-7641. 2009.
Ahn H, Han T. Regulation of TGF-β signaling by PKC depends on Tsc-22 inducibility. Molecular and cellular biochemistry. 360(1-2):47-50. 2012.
Huser CA, Pringle MA, Heath VJ. TSC-22D1 isoforms have opposing roles in mammary epithelial cell survival. Cell death and differentiation. 17(2):304-15. 2010.
Canterini S, Carletti V, Nusca S, Mangia F, Fiorenza MT. Multiple TSC22D4 iso-/phospho-glycoforms display idiosyncratic subcellular localizations and interacting protein partners. The FEBS journal. 280(5):1320-9. 2013.
Mendonça MC, Koles N, Doi SQ, Sellitti DF. Transforming growth factor-beta1 regulation of C-type natriuretic peptide expression in human vascular smooth muscle cells: dependence on TSC22D1. American journal of physiology. Heart and circulatory physiology. 299(6):H2018-27. 2010.

TSC22D1 antibody

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