S-thanatin (Ts) peptide (TFA removed)

S-thanatin (Ts) peptide (TFA removed)

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-GSKKPVPIIYCNRRSGKCQRM-OH (disulfide bond: Cys11-Cys18)

H-Gly-Ser-Lys-Lys-Pro-Val-Pro-Ile-Ile-Tyr-Cys-Asn-Arg-Arg-Ser-Gly-Lys-Cys-Gln-Arg-Met-OH

21

95.12%

C₁₀₂H₁₇₅N₃₅O₂₇S₃

2419.88

Synthetic

Powder

S-thanatin (Ts) peptide is a short antimicrobial peptide (AMP) that exhibits lipopolysaccharide (LPS) binding affinity. The interaction between the Ts peptide and LPS could promote the intercalation of Ts into the cytoplastic membrane, subsequently resulting in a leaky cytoplastic membrane and the disintegration of bacterial respiration and energization. Ts peptide can kill bacteria in a membrane-dependent manner and has been shown to exhibit active antibacterial activity towards numerous gram-negative bacteria, including MDR bacteria. Ts peptide-functionalized levofloxacin-loaded liposomes showed targeted drug delivery to bacteria and exhibited an excellent therapeutic effect in the septic mouse model induced by MDR-KPN.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Fan X, Fan J, Wang X, Wu P, Wu G. S-thanatin functionalized liposome potentially targeting on Klebsiella pneumoniae and its application in sepsis mouse model. Front Pharmacol. 2015

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.