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Product Name
Human Trypsin-3/PRSS3 (His Tag) recombinant protein
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Description
Digestive protease specialized for the degradation of trypsin inhibitors. In the ileum, may be involved in defensin processing, including DEFA5.
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Protein name
Trypsin-3
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Protein short names
MGC176265; TB; RP11-176F3.3; TRY3; TRY4; PRSS4; TRYPSIN-3; MTG; T9; PRSS3
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Uniprot ID
P35030
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Gene Name
PRSS3; PRSS4; TRY3; TRY4
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the human PRSS3 isoform c (P35030-3) (Met 1-Ser 247) was expressed, with a polyhistidine tag at the C-terminus.
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Protein Species
Human
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Molecular weight
The secreted recombinant pro form of the human PRSS3 consists of 243 amino acids and predictes a molecular mass of 26.6 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhPRSS3 is approximately 33 kDa due to glycosylation.
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Purity
> 95 % as determined by SDS-PAGE
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Activity
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (AnaSpec, Catalog#27114). The specific activity is >4,000 pmoles/min/μg.
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Validations
Human Trypsin-3 / PRSS3 Protein (His Tag) SDS-PAGE
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