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Product Name
TRPV6 antibody
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Description
TRPV6 Rabbit Polyclonal antibody. Positive IF detected in Hela cells. Positive IP detected in mouse kidney tissue. Positive WB detected in human placenta tissue, mouse kidney tissue, PC-3 cells. Observed molecular weight by Western-blot: 83 kDa, 70 kDa
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Tested applications
ELISA, IF, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ABP/ZF antibody; Calcium transport protein 1 antibody; CaT L antibody; CaT like antibody; CAT1 antibody; CATL antibody; ECAC2 antibody; Epithelial calcium channel 2 antibody; HSA277909 antibody; LP6728 antibody; TRPV6 antibody; ZFAB antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TRPV6 recombinant protein (Accession Number: BC034814). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IF: 1:10-1:100
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Validations
IP Result of anti-TRPV6 (IP:Catalog No:116419, 4ug; Detection:Catalog No:116419 1:300) with mouse kidney tissue lysate 4000ug.
human placenta tissue were subjected to SDS PAGE followed by western blot with Catalog No:116419(TRPV6 Antibody) at dilution of 1:1000
Immunofluorescent analysis of Hela cells, using TRPV6 antibody Catalog No:116419 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
TRPV6, also named as CAT1 or ECaC2, is a member of the transient receptor potential (TRP) family of membrane proteins. Unlike most TRP channels, TRPV6 and its closest relative, TRPV5, are calcium-selective channels. TRPV6 is highly expressed in the proximal intestine, placenta and exocrine tissues (PMID: 12869611). It is probably involved in calcium absorption in various tissues, including calcium reabsorption in kidney. TRPV6 is overexpressed in some cancers and exhibits oncogenic potential.
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References
- Bolanz KA, Kovacs GG, Landowski CP, Hediger MA. Tamoxifen inhibits TRPV6 activity via estrogen receptor-independent pathways in TRPV6-expressing MCF-7 breast cancer cells. Molecular cancer research : MCR. 7(12):2000-10. 2009.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"